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. 2020 Sep 2;10(9):1552. doi: 10.3390/ani10091552

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

The expression of circRNA-1926 in secondary hair follicle (SHF) bugle of cashmere goats, and its effects on the differentiation of SHF stem cells into hair follicle lineages. (a) Expression analysis of circRNA-1926 in SHF bugle of cashmere goats. (b) Expression analysis of indicator genes in SHF bugle of cashmere goats. (c) Efficiency analysis of circRNA-1926 overexpression in SHF stem cells. (d) CircRNA-1926 overexpression significantly promotes the expression of two indictor genes: keratin 7 and keratin 17 in SHF stem cells. (e) Efficiency analysis of Si-circR1, Si-circR2, and Si-circR3 in knockdown of circRNA-1926, respectively. (f) Si-circRNA1 mediated knockdown of circRNA-1926 significantly decreases the expression of keratin 7 and keratin 17 in SHF stem cells. Blank C = blank cell group, OE-NC = negative control group, and OE-circR = circRNA-1926 overexpression groups. Si-NC = Si-circRNA negative control group. Si-circR1 = Si-circR1 interference group. Si-circR2 = Si-circR2 interference group. Si-circR3 = Si-circR3 interference group. The ns = no significant difference. The asterisk (*) indicates the significant difference (p < 0.05).