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. 2020 Sep 2;10(9):1552. doi: 10.3390/ani10091552

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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CircRNA-1926 promotes the expression of CDK19 in SHF stem cells without changing its promoter methylation. (a) Analysis results of CDK19 mRNA in SHF stem cells treated by circRNA-1926 overexpression assay. (b) Analysis results of CDK19 mRNA in SHF stem cells treated by silnR-1 assay. The asterisk (*) indicates the significant difference (p < 0.05). (c) A graph of CpG islands in the promoter region of the CDK19 gene where the CpG sites are indicated by short pink vertical lines. The nucleotide positions in the CDK19 gene are determined based on the sequence NC_030816.1 in goat genome (https://www.ncbi.nlm.nih.gov/genome/?term=goat). BSP = bisulfite sequencing PCR. (d) Transcription factors having potential binding sites within the BSP amplification region of CDK19 gene promoter. The CpG sites are marked with orange shadow regions. The potential binding sites of transcription factors are marked by blue underlined line along with their corresponding name. (e,f) The BSP results of CDK19 promoter in SHF stem cells treated with circRNA-1926 overexpression or knockdown assay. Filled black circles and unfilled white circles represented the methylated and unmethylated CpG sites, respectively. OE-NC = negative control group, OE-circR = circRNA-1926 overexpression groups, Si-NC = Si-circRNA negative control group, and Si-circR1 = Si-circR1 interference group.

CircRNA-1926 promotes the expression of CDK19 in SHF stem cells without changing its promoter methylation. (a) Analysis results of CDK19 mRNA in SHF stem cells treated by circRNA-1926 overexpression assay. (b) Analysis results of CDK19 mRNA in SHF stem cells treated by silnR-1 assay. The asterisk (*) indicates the significant difference (p < 0.05). (c) A graph of CpG islands in the promoter region of the CDK19 gene where the CpG sites are indicated by short pink vertical lines. The nucleotide positions in the CDK19 gene are determined based on the sequence NC_030816.1 in goat genome (https://www.ncbi.nlm.nih.gov/genome/?term=goat). BSP = bisulfite sequencing PCR. (d) Transcription factors having potential binding sites within the BSP amplification region of CDK19 gene promoter. The CpG sites are marked with orange shadow regions. The potential binding sites of transcription factors are marked by blue underlined line along with their corresponding name. (e,f) The BSP results of CDK19 promoter in SHF stem cells treated with circRNA-1926 overexpression or knockdown assay. Filled black circles and unfilled white circles represented the methylated and unmethylated CpG sites, respectively. OE-NC = negative control group, OE-circR = circRNA-1926 overexpression groups, Si-NC = Si-circRNA negative control group, and Si-circR1 = Si-circR1 interference group.