Skip to main content
. 2020 Aug 22;10(9):1477. doi: 10.3390/ani10091477

Figure 3.

Figure 3

The effect of Fmod overexpression on chicken primary myoblasts differentiation. (a,b) The expression of Fmod mRNA and protein levels after transfection with pcDNA3.1 or Fmod overexpression plasmids for 48 h. (c) The MyoD, MyoG, Mb, and MyHC mRNA expression levels after transfection for 72 h. (d) Western blot analysis of MyoG and MyHC expression levels after transfection for 72 h. (e) Immunofluorescence of chicken myoblasts after transfection for 72 h. MyHc: red; DAPI: blue. (f) Myotube area (%) in myoblasts after transfection for 72 h. Note: * p < 0.05, ** p < 0.01.