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. 2020 Oct 13;6:70. doi: 10.1038/s41421-020-00201-w

Fig. 5. LPS fails to induce the aggregation of wild-type and mutant caspase-11 CARDs.

Fig. 5

a, b Multi-angle light scattering (MALS) profile. The black line indicates the experimentally calculated molecular weight. c Purified caspase-11 WT-CARDaa11–101 protein with a TEV recognition sequence between MBP fusion protein and CARD. Protein (1 mg/mL) was incubated with 5 μg LPS at 4 °C for 4 h. d Purified WT-CARDaa11–101 and VLLV-CARDaa11–101 with MBP tag (1 mg each) incubated with 5 μg LPS at 4 °C for 4 h. e, f Purified WT-CARDaa1–101 and VLLV-CARDaa1–101 with MBP tag (1 mg each) incubated with or without 5 μg LPS at 4 °C for 4 h.