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. 2020 Oct 12;10:16994. doi: 10.1038/s41598-020-73130-z

Table 4.

Stable values of data analyses performed using GeNorm, NormFinder, and BestKeeper.

GeNorm NormFinder BestKeeper
Gene name M Gene name M Gene name SD CV
UBC 0.776 UBC 0.319 UBC 0.53 2.60
TUB 0.797 TUB 0.319 TUB 0.59 2.38
ACT 0.843 ACT 0.431 ACT 0.85 3.92
TUA 0.968 TUA 0.573 TUA 0.86 3.67

M dictates the stability index: the larger the M value, the worse the expression stability of the gene. SD is standard deviation of the Ct value. CV is coefficient of variation of the Ct value. If SD < 1, the gene was considered to be relatively stable; therefore, the smaller the standard deviation and coefficient of variation, the more stable the gene expression.