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. 2020 Oct 12;11:5129. doi: 10.1038/s41467-020-18860-4

Fig. 3. Notch1 activation upregulates Zeb1 expression.

Fig. 3

a Luciferase assays for the wild-type promoter (−1915/+132) of Zeb1 in siNotch1-, siNotch2- or siNotch3-transfected MDA-MB-231 cells. b Relative mRNA levels of Zeb1 in shNotch1-, shNotch2- or shNotch3-transfected MDA-MB-231 cells after treatment with rhJag1. c Protein levels of Zeb1 in shNotch1-, shNotch2- or shNotch3-transfected MDA-MB-231 cells after treatment with rhJag1. d Luciferase assays for wild-type (−1915/+132) or truncated promoters of Zeb1 in MDA-MB-231 cells after treatment with rhJag1. e Luciferase assays for wild-type (−947/+132) or NRE-mutated promoters of Zeb1 in MDA-MB-231 cells after treatment with rhJag1. f, g ChIP assays for recruitment of f NICD and g CBF1 to the endogenous Zeb1 promoter in MDA-MB-231 cells after treatment with rhJag1. Indicated P-values were calculated using two-tailed unpaired Student’s t-test. Data are presented as mean ± SEM in a, b, d–g. Data are representative of three (a–g) independent experiments. Source data are provided as a Source Data file.