Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Oct 12;11:5129. doi: 10.1038/s41467-020-18860-4

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 6 — a Flow cytometry analysis of the CD31⁺ endothelial cell fraction (n = 5 PyMT, 5 PyMT;Zeb1^cKO). b Relative mRNA levels of Jag1 in CD31⁺ endothelial cells (n = 5 PyMT, 5 PyMT;Zeb1^cKO). c Protein levels of NICD and Hes1 in EpCAM⁺ tumor cells (n = 5 PyMT, 5 PyMT;Zeb1^cKO). d Immunofluorescence staining for NICD and CD31 in breast cancer tissue (n = 5 PyMT, 5 PyMT;Zeb1^cKO). The highlighted regions in the left panels are enlarged in the right panels. Scale bars, 50 μm. e Immunofluorescence staining for ALDH1 and CD31 in breast cancer tissue (n = 5 PyMT, 5 PyMT;Zeb1^cKO). Scale bars, 20 μm. f Immunofluorescence staining for CD31 and α-SMA in breast cancer tissue (n = 5 PyMT, 5 PyMT;Zeb1^cKO). Vessel normalization was determined with the CD31/α-SMA ratio. Scale bars, 200 μm. g Dextran leakage assay and immunofluorescence staining for CD31 in breast cancer tissue (n = 5 PyMT, 5 PyMT;Zeb1^cKO). Scale bars, 50 μm. Indicated P-values were calculated using two-tailed unpaired Student’s t-test. Data are presented as mean ± SEM in a, b, d–g. Dots depict individual samples in a, b, d–g. Data are representative of five (a–g) independent experiments. Source data are provided as a Source Data file.