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. 2020 Oct 12;10:16989. doi: 10.1038/s41598-020-73218-6

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Cellular apoptosis by 5FU-SLN₄: Western blot analysis shows the protein expression affected after 48 h of treatment with 12 µM of 5FU-SLN₄ (A). Beta-Actin was used as a loading control. Effects of 5-FU on protein beta-actin ratio and protein expression in HCT-116 cells (B). Effects of 5FU-SLN₄ on protein beta-actin ratio and protein expression in HCT-116 cells (C). Membrane blots were cut to enable blotting for multiple antibodies. Data presented as mean ± SD.