Fig. 3.

SLC2A3 activates GC cell proliferation by activating glycolysis reprogramming. a SLC2A3 protein expression was detected in GES-1 cells and in seven different gastric cancer cell lines. b RT-PCR was used to detect the expression levels of glycolysis enzymes in SLC2A3 overexpression and knockdown cells. c Changes in protein levels were detected by western blot analysis after transfection with SLC2A3 siRNA or SLC2A3 overexpression plasmid. d MKN45 cells transfected with control and SLC2A3 siRNA, or SNU216 cells transfected with empty vector and SLC2A3 overexpression plasmid, were seeded in 24-well plates and exposed to glucose, oligomycin A, 2-DG FCCP, and rotenone to measure extracellular acidification rates (ECAR) and increased oxygen consumption rates (OCR). e Relative lactate release, glucose uptake, and residue glucose from cells was determined by colorimetric analysis. f CCK8 assays were used to assess cell proliferation after siSLC2A3 or SLC2A3 overexpression plasmid transfection. g The effect of glucose deprivation on the growth of cells with overexpression or knock-down of SLC2A3. Cells were cultured in normal and low glucose (0.5 mM) conditions for 48 h, and then subjected to MTT assays. Relative survival was plotted as the percent of cells cultured in normal glucose. Each experiment was performed at least in triplicate and results are presented as mean ± SD. p-values were calculated by one-way ANOVA followed by SNK multiple comparison test. *p < 0.05