Table 1.

Primer sets used in this study

Primer sets for MSP
p14-UM_F	5′-gagtttggttttggaggtgg-3’
p14-UM_R	5′-aaccacaacaacaaacacccct-3’
p14-M_F	5′-tgagtttggttttggaggtgg-3’
p14-M_R	5′-aaaaccacaacgacgaacg-3’
p16-UM_F	5′-ttattagagggtggggtggattgt-3’
p16-UM_R	5′-caaccccaaaccacaaccataa-3’
p16-M_F	5′-ttattagagggtggggcggatcgc-3’
p16-M_R	5′-accccgaaccgcgaccgtaa-3’
Primer sets for MIF polymorphism detection
rs755622_F	5′-tctagccgccaagtggagaaca-3’
rs755622_R	5′-actgtggtcccgccttttgtga-3’
rs5844572_F	5′-tgatccagttgctgccttgtc-3’
rs5844572_R	5′-tccactaatggtaaactcggggac-3’

MSP Methylation specific RCR, UM Unmethylated, M Methylated, F Forward, R Reverse