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. 2020 Oct 13;20:504. doi: 10.1186/s12935-020-01565-5

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — MiR-589-5p restrains cell proliferation, migration and EMT in laryngocarcinoma. a, b CCK8 and colony formation assays examined the repressive impact of overexpressed miR-589-5p on the proliferation of Tu-177 and M4E cells. c, d. Wound healing and transwell assays manifested the hampered capacity of migration in Tu-177 and M4E cells in face of up-regulation of miR-589-5p. Relative wound width in c was calculated using the relative value of wound widths at 24 h to that at 0 h. e IF assessed the augmented expression of E-cadherin and declined level of N-cadherin in Tu-177 and M4E cells with up-regulated miR-589-5p. The standard deviation of control groups in Figure was calculated by 2^−ΔΔCt method as follow: ΔCt (Control) = ΔCt (target, Control) – ΔCt (reference, Control); mean ΔCt (Control) = {ΔCt−1 (Control) + ΔCt−2 (Control) + ΔCt−3 (Control)}/3; ΔΔCt (Control) = ΔCt (Control)−mean ΔCt (Control). **p < 0.01