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. 2020 Jul 6;7(3):71. doi: 10.3390/bioengineering7030071

Figure 3.

Figure 3

Cellular morphology in bioprinted corneal stromal tissue-like structures strongly depends on the material used as bioink. (A) Extrusion bioprinting of human corneal stromal keratocytes (CSKs) in 15% GelMa. Fluorescent staining shows round cells after bioprinting. Scale bars represent 1 mm (left) and 100 µm (right) [88]. (B) Extrusion bioprinting of rat limbal stromal stem cells in PEG-PCL reinforced 15% GelMa. Fluorescent staining shows cellular filopodial extensions up to 50 µm. Scale bars represent 10 mm (left) and 100 µm (right) [87]. (C) Extrusion bioprinting of human turbinate-derived mesenchymal stem cells cultured in CSK differentiation media and embedded in a cornea-derived decellularized ECM bioink. Cells show filopodial extension up to 50 µm. Scale bars represent 5 mm (left) and 200 µm (right) [89]. (D) Laser-based bioprinting of limbal epithelial cells and adipose-derived stem cells in a Matrigel-collagen type-I-based bioink. After bioprinting, cells can spread in the printed substrate. Scale bar represents 1 mm [84]. (E) Drop-on-demand bioprinting of human CSKs in 0.5% agarose–0.2% collagen type-I hybrid bioink. Cells are able to extend their filopodia up to 100 µm. Scale bar represents 100 µm [86].