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. 2020 Sep 29;8:548605. doi: 10.3389/fcell.2020.548605

FIGURE 1.

FIGURE 1

The expression of JMJD3 and SESN2 in human dilated cardiomyopathy and DOX-induced chronic rat cardiomyopathy model. (A,B) The mRNA levels of JMJD3 and SESN2 were measured in the human dilated cardiomyopathy, n = 3 in Normal group, n = 7 in DCM group. SD rats were treated with DOX (accumulate dose 20 mg/kg) for 3 weeks or equal volume of normal saline (NS), n = 6 in NS group, n = 9 in DOX group. (C) The morphologic changes of gross hearts, scale bar = 5 mm. (D,E) Pathological changes of hearts tissues were detected by H&E staining (scale bar = 2 mm) and PSR staining (scale bar = 100 μm). (F) Echocardiographic graphs. (G,H) EF (%) and FS (%) were analyzed. (I,K) The mRNA expression of JMJD3 and SESN2 were measured in the rat hearts by qRT-PCR. (J,L) The protein expression of JMJD3 and SESN2 were detected by Western blot. (M) The protein level of SESN2 was measured in by IHC, scale bar = 100 μm. Data were presented as the mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001 vs. Normal group or NS group. DCM, dilated cardiomyopathy; DOX, doxorubicin; EF, ejection fraction; FS, fractional shortening; HE, hematoxylin-eosin; i.p., intraperitoneally injection; IHC, immunohistochemistry; JMJD3, Jumonji domain-containing 3; NS, normal saline; PSR, Picro Sirius Red; SESN2, Sestrin2; SD, Sprague-Dawley.