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. 2020 Sep 29;8:548605. doi: 10.3389/fcell.2020.548605

FIGURE 2.

FIGURE 2

The expression changes of JMJD3 and SESN2 with chronic DOX-induced cardiotoxicity in NRCMs. NRCMs were incubated with 0.5 μM DOX for indicated time points or with different concentrations of DOX (0.1, 0.5, and 1 μM) for 48 h. (A) Cell morphology of cardiomyocytes was observed by using a light microscopy, scale bar = 100 μm. (B) Hoechst-staining was used to observe chromatin condensation (indicated by arrows), scale bar = 100 μm. (C) Depolarization of mitochondrial membrane potential was detected by TMRE staining, scale bar = 100 μm. (D,E) The protein expression of cleaved caspase3 were measured by Western blot. (F) The mRNA levels of JMJD family were determined by RNA-Seq analysis. (G,H) The mRNA level of JMJD3 was measured by qRT-PCR. (I–K) The protein levels of JMJD3 and SESN2 were measured by Western blot. Data were presented as the mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001 vs. Con group. n = 3. DOX, doxorubicin; NRCMs, primary-cultured of neonatal rat cardiomyocytes; TMRE, tetramethyl rhodamine methyl ester; JMJD, Jumonji domain-containing; JMJD3, Jumonji domain-containing 3; SESN2, Sestrin2.