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. 2020 Sep 29;8:580367. doi: 10.3389/fcell.2020.580367

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Copyright © 2020 Deng, Chen, Liu, Cai, Wan, Zhang, Fan, Zhang and Wang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Knockdown of YTHDF2 impaired goat early embryo development. (A) Schematic diagram of the YTHDF2 knockdown experiment. (B) The expression of YTHDF2 was significantly decreased in YTHDF2 knocked down embryos at the 8-cell stage, as revealed by qPCR. (C) The expression of m⁶A-related genes in YTHDF2 knocked down embryos. (D,E) The developmental rate at 72 h and 168 h after YTHDF2 siRNA injection. (F,G) Representative images of embryos in NC (n = 52) and YTHDF2 knocked down group (n = 71) at 168 h after siRNA injection.