Skip to main content
. 2020 Sep 16;7(3):113. doi: 10.3390/bioengineering7030113

Figure 2.

Figure 2

Expression of oxLDL receptors of monocytes and macrophages cultured in 3D collagen matrices. (A) There is an increase in gene expression of lectin-like oxidized low-density lipoprotein receptor-1 (LOX1) in both monocytes (THP-1) and significantly in all macrophage subtypes when cells are cultured in 3D collagen hydrogels and exposed to oxLDL. Significantly, M1+LDL exhibited the highest fold induction and THP-1+LDL, the lowest, with M0+LDL and M2+LDL comparable. LOX1 expression only changed significantly in M1 with collagen concentration (x-axis, 1 mg/mL to 3 mg/mL). (B) Genetic expression of CD36, another oxLDL receptor, was only measurable in M2+LDL, and significantly higher expression was found in lower density collagen hydrogels. All significances represented as p < 0.05, * compared to untreated sample at the same matrix condition, & between oxLDL samples of different cell types at the same matrix condition, and # significant change with collagen concentration. The dotted horizontal line indicates a level of no change.