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. 2020 Sep 17;10(9):1682. doi: 10.3390/ani10091682

Figure 1.

Figure 1

Diets effect on indices related to protein synthesis of finishing pigs. Relative abundance transcripts of genes (A) Insulin, IR, IGF-1, IGF-1R, MyoD, MyoG, MYF5 and CyclinD1, MAFbx, MURF1, FoxO1 and FoxO4 in longissimus thoracis of finishing pigs. Relative abundance transcripts of genes (B) MyHC-I, MyHC-IIa, MyHC-IIb and MyHC-IIx in longissimus thoracis of finishing pigs. (C) Western blots illustrating the total and phosphorylation level of AKT, mTOR, S6K1 and 4EBP1 in longissimus thoracis. Equal protein loading was confirmed by analysis of β-actin in the protein extracts. (D) The ratios of p-AKT, p-mTOR, p-S6K1 and p-4EBP1 to β-actin. Data are means ± SEM. * p < 0.05, ** p < 0.01. IR, insulin receptor; IGF-1, insulin like growth factor 1; IGF-1R, insulin-like growth factor 1 receptor; FoxO1, forkhead Box O1; FoxO4, forkhead Box O4; MyoD, myoblast determination protein; MyoG, myogenin; MYF5, myogenic factor 5; MAFbx, muscle atrophy F-box; MURF1, Ring finger 1; MyHC-I, myosin heavy chain (MyHC)-I; MyHC-IIa, myosin heavy chain (MyHC)-IIa; MyHC-IIb, myosin heavy chain (MyHC)-IIb; MyHC-IIx, myosin heavy chain (MyHC)-IIx; mTOR, mammalian target of rapamycin; 4EBP1, initiation factor 4E-binding protein 1; S6K1, S6 kinase 1.