. 2020 Sep 17;10(9):1682. doi: 10.3390/ani10091682

Table 2.

Primer sequences used in this study.

Genes	Sequences (5′–3′)		Product Size (bp)	GenBank Accession
MAFbX	Forward	CCCTCTCATTCTGTCACCTTG	104	NM_001044588
MAFbX	Reverse	ATGTGCTCTCCCACCATAGC	104	NM_001044588
MyoG	Forward	CTTCTACCAGGAACCCCACT	230	NM_001012406
MyoG	Reverse	TCCCCAGCCCCTTATCTT	230	NM_001012406
MyoD	Forward	ATGATGACCCGTGTTTCG	383	NM_001002824
MyoD	Reverse	GCCTCGTTGACTTTGCTC	383	NM_001002824
MuRF1	Forward	GCTGGATTGGAAGAAGATGTAT	144	NM_001184756
MuRF1	Reverse	AGGAAAGAATGTGGCAGTGTCT	144	NM_001184756
IGF-1	Forward	TCTTCAGTTCGTGTGCGGAG	165	NM_214256
IGF-1	Reverse	TTGGCAGGCTTGAGGGGT	165	NM_214256
IGF-1R	Forward	ATGGAGGAAGTGACAGGGACTA	116	XM_003361272
IGF-1R	Reverse	GTGGTGGTGGAGGTGAAGTG	116	XM_003361272
Foxo1	Forward	F: CGGCATCATCTTCATCGTC 125	125	NM_214014.2
Foxo1	Reverse	R: CTGTCCTCCCACTCCAGGTA	125	NM_214014.2
Foxo4	Forward	F: CTGTCCTACGCCGACCTCAT 103	103	NM_003135172.2
Foxo4	Reverse	R: TTGCTGTCACCCTTATCCTTG	103	NM_003135172.2
IR	Forward	F: CATACCTGAACGCCAAGAAGTT	100	XM_003123154
IR	Reverse	R: GTCATTCCAAAGTCTCCGATTT	100	XM_003123154
Insulin	Forward	CGCGGCTTCTTCTACAC	134	NM_001109772
Insulin	Reverse	ACGATGCCACGCTTCTG	134	NM_001109772
CyclinD1	Forward	AGGTTGGGAGTGCGTTG	166	XM_021082686.1
CyclinD1	Reverse	TTGGCGGAGATTTGGAG	166	XM_021082686.1
MYF5	Forward	GTTCGGGGACGAGTTTG	272	NM_001278775.1
MYF5	Reverse	GCCTCTGGTTGGGGTTA	272	NM_001278775.1
MyHC-I	Forward	GGCCCCTTCCAGCTTGA	63	L10129
MyHC-I	Reverse	TGGCTGCGCCTTGGTTT	63	L10129
MyHC-IIa	Forward	TTAAAAAGCTCCAAGAACTGTTTCA	100	U11772
MyHC-IIa	Reverse	CCATTTCCTGGTCGGAACTC	100	U11772
MyHC-IIb	Forward	CACTTTAAGTAGTTGTCTGCCTTGAG	80	U90720
MyHC-IIb	Reverse	GGCAGCAGGGCACTAGATGT	80	U90720
MyHC-IIx	Forward	AGCTTCAAGTTCTGCCCCACT	76	U90719
MyHC-IIx	Reverse	GGCTGCGGGTTATTGATGG	76	U90719
β-actin	Forward	CATCGTCCACCGCAAAT	210	NC_010445
β-actin	Reverse	TGTCACCTTCACCGTTCC	210	NC_010445