Figure 1.

H. polygyrus suppresses EAE disease in mice infected on the day of immunization. Female C57BL/6 mice were immunized for EAE on day 0 and were either left untreated (EAE), or received 200 L3 H. polygyrus larvae also on day 0 (EAE + Hp). (A) Disease score (see Methods). (B) Weight change. (C) Spinal cord CD4⁺ T cell infiltration at euthanisation on day 19. (D) Spinal cord macrophage infiltration (CD11b⁺CD45^hi cells as gated in (E) and detailed in Supplementary Figure 1) at euthanisation on day 19. (E) Within the gate of live single leukocytes, CD45⁺ spinal cord cells were further gated into non-myeloid cells (CD45^hiCD11b⁻), microglia (CD45^intCD11b⁺), and infiltrating macrophages (CD45⁺CD11b⁺) as detailed in Supplementary Figure 2. Plots shown are taken from individual mice with median values in (D). Data are pooled from two independent experiments, with a total n = 10, and show arithmetic means and standard errors. Data were analyzed by 2-way ANOVA with Sidak's multiple comparisons test (A,B) or by Mann–Whitney non-parametric test (C,D), *p < 0.05, **p < 0.01.