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. 2020 Sep 29;11:1830. doi: 10.3389/fimmu.2020.01830

Figure 5.

Figure 5

Early, but not chronic, H. polygyrus infection is required to inhibit EAE. H. polygyrus infection was administered 28 days prior to, or on the day of EAE induction. Female C57BL/6 mice were immunized for EAE on day 0 and were either left untreated (EAE), or received 200 L3 H. polygyrus larvae also on day 0 (EAE + Hp D0), or on day−28 (EAE + Hp D-28) prior to EAE immunization. (A) Disease course in mice with EAE with or without H. polygyrus infection at d0 or day−28. (B) Total cell numbers in inguinal lymph nodes at day 19. (C) Foxp3+ cell frequencies in inguinal lymph node. (D) MOG-specific responses of spleen cells, IL-17A measured by ELISA on day 19. (E) MOG-specific responses of spleen cells, IL-4. All data are pooled from two independent experiments, with a total n = 10, and shown are the means ± SEM; (A) was tested by mixed-effects analysis with a multiple comparisons post-tests. (B,C) were tested by 1-way ANOVA with Tukey's multiple comparisons tests. (D,E) were tested by 2-way ANOVA with Bonferroni's multiple comparisons tests. Shown are the means ± SEM *p < 0.05, **p < 0.01.