Figure 6.

H. polygyrus fails to protect against EAE in IL-4Rα-deficient mice. Female C57BL/6 or IL-4Rα-deficient mice were immunized for EAE on day 0 and were either left untreated or received 200 L3 H. polygyrus larvae also on day 0 (+Hp). (A) Disease course of EAE in uninfected and infected C57BL/6 wild-type and IL-4Rα-deficient mice. (B) Adult worm burdens in infected C57BL/6 wild-type and IL-4Rα-deficient mice at the time of euthanisation, day 26. (C) Frequency of CD4⁺ T cells expressing the Th2 marker, GATA3, in spleen at day 25 as measured using flow cytometry. (D) Frequency of CD4⁺ T cells expressing ST2 in spleen. (E) Frequency of CD4⁺ T cells expressing PD1 in spleen. (F) Frequency of Foxp3⁺ Tregs in the spleen. Data are from one of two similar independent experiments with n = 4–6 per group; (A) was tested by a 2-way ANOVA with Bonferroni's multiple comparisons test. (C–F) were statistically analyzed by unpaired t-tests with Welch's correction if SDs were not equal. Shown are the means ± SEM and *p < 0.05 and ****p < 0.0001.