Figure 7. CNR2 was a target of miR-545-3p. A, The putative binding sites for miR-545-3p and CNR2 3'UTR are shown. B, Dual-luciferase reporter assay was conducted to confirm the relationship between miR-545-3p and CNR2. C, RIP assay was utilized to validate the correlation between miR-545-3p and CNR2. D, The protein expression of CNR2 was examined in hFOB1.19 cells transfected with negative control (NC), miR-545-3p, anti-NC, or anti-miR-545-3p. E, After transfection with vector, TUG1, si-NC, or si-TUG1, CNR2 expression was tested by western blot assay. Data are reported as means±SD of three independent experiments. *P<0.05 (t-test).
