Figure 8. MiR-545-3p inversed the effect of CNR2 on osteogenic differentiation. A, The protein level of CNR2 was measured in hFOB1.19 cells transfected with si-NC (negative control), si-CNR2, anti-NC+si-CNR2, or anti-miR-545-3p+si-CNR2. Additionally, CNR2 expression was detected in hFOB1.19 cells introduced with vector, CNR2, NC+CNR2, or miR-545-3p+CNR2. B, ALP activity was determined in osteogenic differentiated hFOB1.19 cells transfected with si-CNR2 and/or anti-miR-545-3p. C, After introduction with CNR2 and/or miR-545-3p, ALP activity was evaluated in osteogenic differentiated hFOB1.19 cells. D, Protein levels of ALP, Runx2, osteocalcin (OCN), and osteopontin (OPN) were measured in osteogenic differentiated hFOB1.19 cells following CNR2 down-regulation and/or miR-545-3p down-regulation. E, Expression of ALP, Runx2, OCN, and OPN was detected in osteogenic differentiated hFOB1.19 cells following CNR2 overexpression and/or miR-545-3p overexpression. Data are reported as means±SD of three independent experiments. *P<0.05 (ANOVA).