Table 1.
SARS CoV-2 detection. Air samples and purified pollen, freshly collected in Leipzig or purchased from Allergon were tested for a SARS-CoV-2 specific signal via RT-PCR on day 0 and day 8 as well as tested for Virus-induced cytopathic effects (CPE) on Vero cells.
| Sample # | Sample description | SARS-CoV-2 PCR day 0a | Virus-induced CPE | SARS-CoV-2 PCR day 8 p.i.b |
|---|---|---|---|---|
| 120 | Week 11.03.-26.03.20 (Air sample) | Negativec | None | Negative |
| 121 | Week 26.03.-02.04.20 (Air sample) | Negative | None | Negative |
| 122 | Week 02.-09.04.20 (Air sample) | Negative | None | Negative |
| 123 | Week 09.-16.04.20 (Air sample) | Negative | None | Negative |
| 124 | Week 16.-23.04.20 (Air sample) | Negative | None | Negative |
| 127 | Week 07.05.-14.05.20 (Air sample) | Negative | None | Negative |
| 128 | Week 14.05.-21.05.20 (Air sample) | Negative | None | Negative |
| 129 | Week 21.05.-28.05.20 (Air sample) | Negative | None | Negative |
| 610 | Betula pendula (purified fresh pollen from Leipzig) | Negative | None | Negative |
| 631 | Ostrya carpinifolia (purified fresh pollen from Leipzig) | Negative | None | Negative |
| 673 | Quercus robur (purified fresh pollen from Leipzig) | Negative | None | Negative |
| A | Alnus glutinosa (Allergon) | Negative | None | Negative |
| B | Betula pendula (Allergon) | Negative | None | Negative |
| C | Corylus avellana (Allergon) | Negative | None | Negative |
| Ad | Alnus glutinosa (Allergon) | Negative | None | Negative |
| Bd | Betula pendula (Allergon) | Negative | None | Negative |
| Cd | Corylus avellana (Allergon) | Negative | None | Negative |
a
SARS-CoV-2 PCR; measured without prior cell cultivation, day 0.
b
SARS-CoV-2 PCR; measured after cultivation for 8 days.
c
Negative: <10 genome equivalents.
d
Preincubated with SARS-CoV-2 (3 × 104 TCID50) before extensive washing.