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. 2020 Oct 13;755:142881. doi: 10.1016/j.scitotenv.2020.142881

Table A2.

SARS CoV-2 detection – positive controls. Air samples from sampling period 02.-09.04.2020 (sample 122), 09.-16.04.2020 (sample 123) and 16.-23.04-2020 (sample 124) were spiked with a) 1 × 107 and b) 1 × 104 SARS-CoV-2 genome equivalents (Geq) to exclude a potential interference of sample matrix to Geq detection. * SARS-CoV-2 PCR; E, RdRp and N gene, measured without prior cell cultivation, day 0; ** SARS-CoV-2 PCR; E, RdRp and N-Gen, measured after cultivation for 8 days.

Sample # Characteristics Spiked with SARS-CoV-2 (Geq) SARS-CoV-2 PCR (Geq) day 0a Virus-induced CPE SARS-CoV-2 PCR (Geq) day 8b
122a Week 02.-09.04. (Air sample) 1 × 107 0.91 × 107 +++ >1 × 108
122b Week 02.-09.04. (Air sample) 1 × 104 1.24 × 104 ++ 2.1 × 107
123a Week 09.-16.04. (Air sample) 1 × 107 0.96 × 107 +++ >1 × 108
123b Week 09.-16.04. (Air sample) 1 × 104 0.98 × 104 ++ 9.7 × 107
124a Week 16.-23.04. (Air sample) 1 × 107 0.97 × 107 +++ >1 × 108
124b Week 16.-23.04. (Air sample) 1 × 104 0.98 × 104 ++ 5.7 × 107

Note: there was no inhibition of SARS-CoV-2 replication.

a

SARS-CoV-2 PCR; E, RdRp and N gene, measured without prior cell cultivation, day 0.

b

SARS-CoV-2 PCR; E, RdRp and N-Gen, measured after cultivation for 8 days.