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[Preprint]. 2020 Oct 7:2020.05.04.077826. [Version 2] doi: 10.1101/2020.05.04.077826

Figure 1. Measurements of TMPRSS2 activity in transfected cells.

Figure 1.

A. Western blot of TMPRSS2 protein expression in HEK-293T cells transfected with PLX304 vector or PLX304-TMPRSS2 vector is shown. TMPRSS2-containing V5 tag was assessed by anti-V5 antibody, and anti-GAPDH antibody was used as a loading control. B. Arbitrary fluorescence unit (AFU) measurements of control or TMPRSS-overexpressing cells incubated with BOC-QAR-AMC for 75 minutes at 37 °C are shown. Wells containing PBS and BOC-QAR-AMC were used as background fluorescence reads. C. Fluorescence of control or TMPRSS2-overexpressing cells was measured every 15 minutes for a total time of 180 minutes. The average proteolytic activity rate per minute (D) or hour (E) of control or TMPRSS2-overexpressing cells. The fluorescent signal was measured by the UV filter (excitation 365 nm and emission 410 nm). Data in B, D, and E represent the mean ± SD with interquartile ranges in B, D and E. GAPDH, glyceraldehyde-3-phosphate dehydrogenase; TMPRSS2, transmembrane serine protease 2.