Skip to main content
[Preprint]. 2020 Oct 11:2020.10.09.334128. [Version 2] doi: 10.1101/2020.10.09.334128

Figure 3. Functional TMEM41B is conserved across mammalian and vector species.

Figure 3.

(A) Graphical representation of human TMEM41B isoforms and deletion construct. VTT domain is shaded pink. Amino acid length of each isoform is indicated. Isoform 1, UniProt ID: Q5BJD5–1; isoform 2, UniProt ID: Q5BJD5–2; isoform 3, UniProt ID: Q5BJD5–3; isoform 4, UniProt ID: E9PJ42. (B) WT, TMEM41B KO, and TMEM41B KO cells expressing human TMEM41B isoforms or (C) mosquito and tick TMEM41B isoforms infected with YFV Asibi, MOI = 0.1 PFU/cell for 72 h, or ZIKV, MOI = 0.25 PFU/cell for 48 h. (D) Table comparing homology between human TMEM41B (isoform 1) and mosquito and tick TMEM41B homologues. Numbers indicate percent amino acids identity for the full-length protein (first number) vs VTT domain only (second number). Protein sequences were aligned using Geneious Software (Geneious 8.1.9. https://www.geneious.com). Cells were analyzed by flow cytometry and plotted as a percentage of viral antigen positive cells. Error bars depict SD for three replicates.