Fig. 2.

Changes in the metabolomics of TCD-BM and TCD-BM+T cells groups.

(a, b) A comparison was made between detected metabolites with paired Student's t-test and then Bonferroni correction. The volcano plot shows the variation in the number of metabolites between TCD-BM (n=6) and TCD-BM+T cells (n=6) groups according to the –log (q value) and log2 (fold change) on days 14 (a) and 28 (b). (c) The most important 50 metabolites after Student's t-test and the hierarchical clustering of samples shown in the heatmap. (d) KEGG-annotated metabolic pathways between TCD-BM and TCD-BM+T cells groups. (e) On day 14, the heatmap summarizes the altered fecal metabolites related to tyrosine between TCD-BM and TCD-BM+T cells groups and only displays the decrease of tyrosine in the TCD-BM+T cells group (two-tailed Student's t-test, p<0.01). (f) On day 28, the TCD-BM+T cells group showed a significant decrease in tyrosine, L-dopa, L-aspartic acid, L-glutamic acid and S-adenosylhomocysteine (two-tailed Student's t-test, p<0.01). (g, h) Correlation network between altered metabolites (circular) and differentially abundant microbiota (square). A connection means that a microbe is correlated with a metabolite; red and blue lines indicate positive and negative correlations respectively; volume size refers to the strength of the correlation. Networks between TCD-BM and TCD-BM+T cells groups were shown on days 14 (g) and 28 (h). The number of mice in each group was indicated.