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. 2020 Oct 1;16(10):e1008461. doi: 10.1371/journal.ppat.1008461

Fig 7. Co-stimulation with IFNα and IL1β enhances IFNα-induced gene expression in primary human hepatocytes and viral clearance of HCV in a replicon cell line.

Fig 7

(A) Effect of co-stimulation with IFNα and IL1β on mRNA expression of IFNα-induced genes in primary human hepatocytes. Cells were stimulated with 500 U/ml IFNα alone or co-treated with 500 U/ml IFNα and 10 ng/ml IL1β. RNA was extracted at the indicated time points and analyzed by qRT-PCR. Triplicate samples were analyzed from three different donors and error bars represent SD. (B) Scheme of the bicistronic subgenomic HCV reporter RNA (replicon). IRES, internal ribosomal entry site; NTR, non-translated region. (C) Enhanced suppression of HCV replication in cells co-stimulated with IFNα and IL1β. Huh7/HCV/Luc replicon cells were stimulated with 50 U/ml IFNα alone or were co-treated with 50 U/ml IFNα and 1 ng/ml IL1β. The values are relative to the unstimulated control. Error bars represent the SEM of six technical replicates.