Fig 6. rhRod co-localizes with P-selectin and attenuates neutrophil accumulation in the liver sinusoids in endotoxemic mice.

(A) Montage image from intravital microscopy of mouse liver sinusoids after intraperitoneal Atto 550-labeled rhRod (top) or control (bottom) followed by endotoxin injection. rhRod (green) and P-selectin (red) colocalize (yellow) along the sinusoid wall. Neutrophils were labeled with anti-Ly-6G antibody (cyan) and the sinusoids were identified using 150,000 Da-Dextran (gray). (B) rhRod administration decreased the number of firmly adherent (<1 cell body movement over 1 minute) neutrophils per vessel area in liver sinusoids. (C) Representative images of vimentin expression (brown; black arrows) with hematoxylin counterstain in the liver of mice 4 hours after saline or endotoxin intraperitoneal injection. (D) There was no difference in the percent area of vimentin staining between the two groups. Scale bar = 50 μm. *p<0.05.