Table 1. KD determination by biolayer interferometry.
Sensor | Ligand | Analyte | KD (nM) | R2 |
---|---|---|---|---|
AR2G | rhVim | P-sel/Fc | 60 ± 12 | 0.9562 |
rhVim | PSGL-1/Fc | ND | 0 | |
rhRod | P-sel/Fc | 41 ± 6 | 0.955 | |
rhRod | PSGL-1/Fc | ND | 0 | |
AHC | P-sel/Fc | rhVim | 110 ± 19 | 0.9739 |
P-sel/Fc | rhRod | 10 ± 0.6 | 0.9911 | |
PSGL-1/Fc | rhVim | ND | 0 | |
PSGL-1/Fc | rhRod | ND | 0 |
AR2G: amine reactive 2nd generation sensor; AHC: anti-human IgG capture sensor; ND: not determined. R2<0.8 was determined to be indeterminate.