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. 2020 Oct 13;15(10):e0240164. doi: 10.1371/journal.pone.0240164

Table 1. KD determination by biolayer interferometry.

Sensor Ligand Analyte KD (nM) R2
AR2G rhVim P-sel/Fc 60 ± 12 0.9562
rhVim PSGL-1/Fc ND 0
rhRod P-sel/Fc 41 ± 6 0.955
rhRod PSGL-1/Fc ND 0
AHC P-sel/Fc rhVim 110 ± 19 0.9739
P-sel/Fc rhRod 10 ± 0.6 0.9911
PSGL-1/Fc rhVim ND 0
PSGL-1/Fc rhRod ND 0

AR2G: amine reactive 2nd generation sensor; AHC: anti-human IgG capture sensor; ND: not determined. R2<0.8 was determined to be indeterminate.