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. 2020 Aug 31;11(19):3130–3143. doi: 10.1021/acschemneuro.0c00477

Figure 2.

Figure 2

Curve-shift GIRK activation assay of D2R antagonism upon coapplication of SV-III-130 and DA. A) Assay principles; 1 μM DA elicits a full agonist response (left, red arrow). The subsequent response amplitude in the presence of variable concentrations of SV-III-130 and DA, following 500 s coapplication (right, green arrow), was normalized to the control response elicited by 1 μM DA. In the example shown, 1 μM SV-III-130 coapplied with 100 μM DA; n = 3 oocytes. Thick lines represent mean normalized currents, whereas thin lines indicate SEM. B) Current amplitude at the end of the 500 s coapplication period, normalized to the instantaneous maximum response amplitude in the same oocyte and plotted against DA concentration, for varying concentrations of SV-III-130 or control. n = 3–7 oocytes per data point. Data shown are means ± SEM.