Figure 3.

Propofol mitigates cell tumorigenesis and aerobic glycolysis in lung cancer by regulating circTADA2A. A549 and H1299 cells were transfected with pcDNA-NC or pcDNA-circTADA2A prior to propofol exposure. (a) The transfection efficiency was determined using qRT-PCR. (b,c) Cell proliferation was analyzed using CCK-8 and BrdU assay. (d,e) Transwell assay was applied to detect cell migration and invasion. (f,g) The glucose consumption and lactate production were assessed using a glucose and lactate assay kit. (h) Extracellular acidification measurements were conducted by Seahorse XF24 Extracellular Flux Analyzer. (I. J) The levels of GLUT1, PKM2, HK2, and LDHA protein in A549 and H1299 cells were examined by western blot. *P < 0.05.