Figure 4.

CircTADA2A is a sponge of miR-455-3p in lung cancer cells. (a) The potential binding sites between circTADA2A and miR-455-3p were predicted by StarBase3.0 program. (b,c) Luciferase activity of A549 and H1299 cells co-transfected with circTADA2A-WT or circTADA2A-MUT and miR-455-3p mimics or miR-NC was examined by dual-luciferase reporter assay. (d,e) qRT-PCR analysis of miR-455-3p expression in A549 and H1299 cells transfected with si-NC, si-circTADA2A, pcDNA-circTADA2A, or pcDNA-NC was conducted. (f,g) The expression of miR-455-3p in lung cancer tumor tissues and normal tissues, as well as lung cancer cell lines and normal BEAS-2B cell line was measured using qRT-PCR. (h) The level of miR-455-3p was examined using qRT-PCR in A549 and H1299 cells treated with propofol. *P < 0.05.