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. 2020 Aug 28;19(19):2538–2552. doi: 10.1080/15384101.2020.1810393

Figure 5.

Figure 5.

Propofol performs anti-tumor effects by regulating circTADA2A/miR-455-3p axis in lung cancer cells. A549 and H1299 cells were transfected with pcDNA-NC, pcDNA-circTADA2A, pcDNA-circTADA2A + miR-NC, or pcDNA-circTADA2A + miR-455-3p before propofol treatment. (a) The transfection efficiency was analyzed using qRT-PCR. (b,c) Cell proliferation was analyzed using CCK-8 and BrdU assay. (d,e) Cell migration and invasion were detected by transwell assay. (f,g) The glucose consumption and lactate production were examined with a glucose and lactate assay kit. (h) Seahorse XF24 Extracellular Flux Analyzer was applied to measure extracellular acidification. (i,j) The levels of GLUT1, PKM2, HK2, and LDHA protein in A549 and H1299 cells were examined by western blot. *P < 0.05.