Figure 7.

Propofol inhibits cell tumorigenesis and aerobic glycolysis by regulating miR-455-3p/FOXM1 axis in lung cancer. A549 and H1299 cells transfected with anti-miR-NC, anti-miR-455-3p, anti-miR-455-3p + si-NC, anti-miR-455-3p + si-FOXM1 before propofol treatment. (a,b) The analysis of A549 and H1299 cell proliferation was conducted by CCK-8 and BrdU assay. (c,d) The migrated and invaded A549 and H1299 cells were measured using transwell assay. (e,f) The glucose consumption and lactate production were examined with a glucose and lactate assay kit. (g) Extracellular acidification measurements were carried out by Seahorse XF24 Extracellular Flux Analyzer. (h,i) Western blot analysis of GLUT1, PKM2, HK2, and LDHA protein in A549 and H1299 cells was conducted. *P < 0.05.