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. 2020 Sep 15;19(19):2496–2508. doi: 10.1080/15384101.2020.1807670

Figure 2.

Figure 2.

miR-7 is down-regulated and Raf-1 is up-regulated in GC cells; Raf-1 is a target gene of miR-7. (a). The relative expression of miR-7 in human normal gastric mucosal epithelial cell line RGM-1 and GC cell lines; (b). The relative expression of Raf-1 mRNA in human normal gastric mucosal epithelial cell line RGM-1 and GC cell lines; (c). Raf-1 protein bands and protein expression in human normal gastric mucosal epithelial cell line RGM-1 and GC cell lines; (d)&(f). The binding site of Raf-1 and miR-7 predicted by bioinformatics website; (e)&(g). The binding relationship between miR-7 and Raf-1 analyzed by dual luciferase reporter gene assay; (h). The relative expression of miR-7 after transfection in each group of cells; (i). The relative expression of Raf-1 mRNA after transfection in each group of cells; (j). The protein bands and protein expression of Raf-1 after transfection in each group of cells; $ vs the normal gastric mucosal epithelial cells RGM-1, P < 0.05; * vs the mimics NC group, P < 0.05; & vs the si-NC group, P < 0.05; # vs the miR-7 inhibitors + si-NC group, P < 0.05; Measurement data were mean ± standard deviation of three independent experiments. One-way ANOVA was utilized for data analysis among multiple groups, followed by Tukey’s multiple comparisons test.