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. 2020 Mar 2;16(9):2245–2251. doi: 10.1080/21645515.2020.1721223

Table 1.

Purified H7N9 influenza whole-virus standard antigens identified by mass spectrometry.

Protein name LC-MS/MS
Accession number Protein hit No. of observed peptidesb Mascot scorec
PB1 gi | 525338806 Influenza A virus (A/duck/Anhui/SC702/2013 [H7N9]) 13 42 | 23
NP gi | 256259586 Influenza A virus (Puerto Rico/8/1934) 2 430 | 1
NA gi | 459252891 Influenza A virus (A/duck/Anhui/SC702/2013 [H7N9]) 12 334 | 1
HA subunit 1 gi | 475662454 Influenza A virus (A/duck/Anhui/SC702/2013 [H7N9]) 7 476 | 45
PNGase F gi | 157833480 Glycosylasparaginase from Flavobacterium meningosepticum 15 602 | 57
Matrix protein 1 gi | 8486123 Influenza A virus (A/Puerto Rico/8/34 [H1N1]) 4 150 | 1
HA subunit 2 gi | 475662454 Influenza A virus (A/duck/Anhui/SC702/2013 [H7N9]) 5 412 | 59

aThe masses of proteins in SDS-PAGE gels (Figure 1) were calculated using TotalLab quantification software.

bThe observed peptides include all peptides that showed differences in sequence, modification or charge.

cThe protein hit is the protein with the highest protein score.

dThe Mascot score was used to identify the protein with the highest score, and the number after “|” indicates the significant protein score (p < 0.05).