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. 2020 Aug 12;16(9):2042–2050. doi: 10.1080/21645515.2020.1793711

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© 2020 The Author(s). Published with license by Taylor & Francis Group, LLC.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License (http://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited, and is not altered, transformed, or built upon in any way.

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Figure 1. — Vaccine preparation. OPT1 H7 rHA was designed by substitution of 3 amino acids of wild-type H7 rHA(A). This modified HA sequence was expressed as a trimeric soluble recombinant HA protein (H7 rHA) by replacing the transmembrane domain with a trimerization domain (B). The expression of H7 rHA was confirmed by anti-his taq western blot analysis (C) and native blue PAGE was conduced to confirm the trimeric forms (D) WT: WT H7 rHA, OPT1: OPT1 H7 rHA,, TM*: transmembrane domain, Std: protein standard Expected molecular weight of monomeric H7 rHA = 65 kDa, Expected molecular weight of trimeric H7 rHA = 194.8 kDa.