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. 2020 Jun 19;177(1):281–299. doi: 10.1093/toxsci/kfaa088

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© The Author(s) 2020. Published by Oxford University Press on behalf of the Society of Toxicology.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs licence (http://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial reproduction and distribution of the work, in any medium, provided the original work is not altered or transformed in any way, and that the work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 7. — Activation of nuclear factor erythroid 2-related factor 2 (NRF2) by acetaminophen (APAP) and ticlopidine is dependent on CYP450-mediated metabolism and by bardoxolone in a CYP450-independent mechanism. HEK293 cells expressing CYP2E1, CYP2C9, or CYP3A4 were exposed to increasing concentrations of (A) APAP, (B) ticlopidine, and (C) bardoxolone, respectively, for 6 h. Whole cell lysates were prepared, subjected to SDS-PAGE and analyzed by immunoblotting with anti-NRF2, anti-Flag (for CYP450), and anti-GAPDH antibodies. Abbreviations: BDX, bardoxolone; Dox, doxycycline.