Figure 9.

Bruton tyrosine kinase case study. A, MRK-A, B, or C (10 µm) was incubated in rat and human liver microsomes with added trapping agents (glutathione 5 mM, semicarbazide 5 mM, potassium cyanide 1 mM) at 37°C for 1 h. The assays were then quenched with acetonitrile, the debris precipitated, and the supernatant concentrated and analyzed by LC-MS. B, The amounts of metabolites represented are calculated based on mass spectral ionization efficiency, assuming similar response across metabolites.