Figure 1.

Experimental design of the study and summary of data. A, 90-day-old female conventional (CV) and germ-free (GF) mice were orally exposed to the Fox River polychlorinated biphenyl (PCB) mixture (6 or 30 mg/kg) or corn oil (vehicle control, 10 ml/kg). Twenty-four hours the final dosing, organs were collected. RNA-seq, 16s rDNA-seq, LC-MS were performed on the liver, large intestinal pellets, and liver and serum, respectively. B, Principal component analysis of the liver transcriptome and liver and serum metabolome. Individual points on the principal components scale are labeled by enterotype and exposure. C, Venn diagrams of differentially expressed genes of the liver and differentially abundant metabolites in the liver and serum.