Figure 1.

Schematic description of the hybrid development utilizing CRISPR/Cas genome editing system. The two line hybrid development through knockout of P/TGMS = photoperiod/thermo-sensitive genic male sterile genes from elite cultivar. The selection of target in any exon of gene under consideration and later targeted modification through CRISPR/Cas technology helps to induce mutations. The frame shift mutation (Deletion and Insertion) is mostly desirable. The screening of mutants to get male sterile plants, crossing with compatible restorer generate F₁ hybrid.