Skip to main content
. Author manuscript; available in PMC: 2020 Oct 13.
Published in final edited form as: Virology. 2019 Oct 18;539:26–37. doi: 10.1016/j.virol.2019.10.003

Figure 3. MACS column-enrichment of NK cells and lysis of differentiated T cell cultures.

Figure 3.

NK cells were partially purified from spleen leukocytes from day 3 LCMV-infected C57BL/6 mice and tested in 4-hour 51Cr-release cytotoxicity assays against Th1 and Th2 cultures. A. Upper panels are low cytometry plots against NK1.1 (y-axis) and NKp46 (x-axis). Boxes in upper panels represent %NK1.1+ cells. B. Unfractionated cytotoxicity data are plotted at E:T = 100:1, whereas enriched and depleted samples are plotted at E:T =10:1.