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. 2020 Oct 9;13:10087–10096. doi: 10.2147/OTT.S275218

Figure 2.

Figure 2

Circ-ELF2 promotes GM cell progression in vitro. (A) Circ-ELF2 expression was detected after transfection in LN229 cells by qRT-PCR. (B) Circ-ELF2 expression was detected after transfection in U87MG cells by qRT-PCR. (C, D) CCK-8 assay was used to detect the viability of LN229 and U87MG cells after circ-ELF2 silencing/overexpression. (E, F) Colony formation assay was used to detect the clone-forming ability of LN229 and U87MG cells after circ-ELF2 silencing/overexpression. (G, H) AO/EB staining assay was used to detect the apoptosis of LN229 and U87MG cells after circ-ELF2 silencing/overexpression. (I, J) Flow cytometric analysis was used to detect the apoptosis of LN229 and U87MG cells after circ-ELF2 silencing/overexpression. (K, L) Wound healing assay was used to detect the migration of LN229 and U87MG cells after circ-ELF2 silencing/overexpression. (M, N) Transwell migration and invasion assay was used to detect the migration/invasion of LN229 and U87MG cells after circ-ELF2 silencing/overexpression. *P < 0.05, **P < 0.01.