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. 2020 Oct 9;13:10087–10096. doi: 10.2147/OTT.S275218

Figure 4.

Figure 4

MUC15 is a downstream target of miR-510-5p. (A) Relative MUC15 mRNA expression was determined after transfection with sh-circ-ELF2-1 or sh-NC in LN229 and U87MG cells by qRT-PCR. (B, C) Relative MUC15 mRNA and protein expression was determined after transfection in LN229 and U87MG cells by qRT-PCR and immunoblotting, respectively. (D) Relative MUC15 mRNA expression in GM/normal tissues analyzed by qRT-PCR. (E) Pearson correlation analysis of MUC15 mRNA and miR-510-5p expression in 25 paired GM tissues. (F) Pearson correlation analysis of MUC15 mRNA and circ-ELF2 expression in 25 paired GM tissues. (G) Schematic illustration of MUC15 3ʹ-UTR-wt and MUC15 3ʹ-UTR-mut luciferase reporter vectors. (H) The binding ability between MUC15 3ʹ-UTR and miR-510-5p was measured by dual-luciferase reporter assay in 293T cells. *P < 0.05, **P < 0.01.