Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Oct 9;12:9915–9928. doi: 10.2147/CMAR.S273687

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2020 Liu et al.

This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php).

PMC Copyright notice

HMGA1 upregulation reverses the impacts of si-VPS9D1-AS1 on CRC cells. (A) Western blotting was used to assess the protein level of HMGA1 in HCT116 and SW480 cells after pcDNA3.1-HMGA1 or pcDNA3.1 introduction. (B, C) pcDNA3.1-HMGA1 or pcDNA3.1, alongside si-VPS9D1-AS1, was transfected into HCT116 and SW480 cells, followed by the measurement of proliferation and apoptosis using CCK-8 and flow cytometry assays. (D, E) Cell migration and invasion assays were performed to determine the migratory and invasive abilities of the cells mentioned above. *P < 0.05 and **P < 0.01.

Abbreviations: HMGA1, high-mobility group AT-hook 1; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; VPS9D1-AS1, VPS9D1 antisense RNA 1; NC, negative control; miR-525-5p, microRNA-525-5p; si-VPS9D1-AS1, small interfering RNA targeting VPS9D1-AS1; si-NC, negative control small interfering RNA; PI, propidium iodide.