Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Gene (Mus musculus) | Braf | Mouse Genome Informatics (MGI) | MGI:88190 | |
| Gene (M. musculus) | Tyr::CreER | MGI | MGI:3641203 | MGI Transgene name: GeneTg(Tyr-cre/ERT2)13Bos |
| Genetic reagent (M. musculus) | B6.Cg-Tg(Tyr-cre/ERT2)13Bos Braftm1Mmcm /BosJ | Dankort et al., 2009 | RRID:MGI:5902125 | |
| Antibody | Anti-Pmel (rabbit monoclonal) | Abcam | Cat#ab137078 RRID:AB_2732921 |
Also known as anti-melanoma gp100 IF (1:500) |
| Antibody | Anti-BrdU (rat monoclonal) | Abcam | Cat#ab6326 RRID:AB_305426 |
IF (1:500) |
| Antibody | Goat anti-rabbit alexa fluor 594 conjugated (polyclonal) | ThermoFisher Scientific | Cat#A-11012 RRID:AB_2534079 |
(1:2000) |
| Antibody | Chicken anti-rat alexa fluor 488 (polyclonal) | ThermoFisher Scientific | Cat#A-21470 RRIB:AB_2535873 |
(1:2000) |
| Sequence-based reagent | Braf_F | IDT | PCR primer | 5’-TGAGTATTTTTGTGGCAACTGC −3’ |
| Sequence-based reagent | Braf_R | IDT | PCR primer | 5’-CTCTGCTGGGAAAGCGCC −3’ |
| Sequence-based reagent | Cre_F | IDT | PCR primer | 5’- GGTGTCCAATTTACTGACCGTACA-3’ |
| Sequence-based reagent | Cre_R | IDT | PCR primer | 5’- CGGATCCGCCGCATAACCAGTG −3’ |
| Chemical compound, drug | 4-hydroxytamoxifen | Sigma-Aldrich | Cat#68047-06-3 | |
| Chemical compound, drug | TrueBlack lipofuscin | Biotium | Cat#23007 | |
| Software, algorithm | Mathematica | Wolfram | RRID:SCR_014448 | |
| Software, algorithm | Scanpy | Wolf et al., 2018 | RRID:SCR_018139 | |
| Software, algorithm | Cell Ranger | 10X genomics | RRID:SCR_017344 | |
| Software, algorithm | CompuCell3D | Swat et al., 2012 | RRID:SCR_003052 |