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. 2020 Oct 13;10:17063. doi: 10.1038/s41598-020-73391-8

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Collection location and physiological tests. (A) Queens for laboratory colony rearing were collected from sites near the spatial and bioclimatic extremes of the Bombus vosnesenskii range from relatively low and high elevations in southern CA and northern OR (Table S1). The map was created in ESRI ArcMap 10.8, with state outlines and digital elevation data obtained from https://www.diva-gis.org/Data. The B. vosnesenskii range outline was taken from the species distribution model published perviously^3,33. (B) Mean monthly temperatures over the course of a year for queen collecting sites. (C) The CT_MIN temperature of laboratory reared workers is strongly correlated with the annual mean temperature of collection sites of the founding queens. The coastal L-CA colony discussed in Results is indicated by a black dot in each panel.