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. 2020 Oct 13;10:17063. doi: 10.1038/s41598-020-73391-8

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Gene Ontology enrichment analysis of edgeR/limma and WCGNA results. Significantly enriched gene ontology terms identified in edgeR/limma are listed on the rows for different contrasts, with information on the WCGNA modules (1–6) that are also enriched for that term. Each panel has a different main factor for the contrast (top label), with GO enrichment in genes with decreased or increased expression in the main factor relative to the contrast factor (bottom label). (A) Treatment comparisons (control and CT_MIN) against CT_MAX treatment, presenting full GO analysis results (Dataset S2) reduced with ReViGO and further filtered to include common terms from other thermal stress studies for the purposes of presentation clarity. (B) Region comparisons (L-CA, H-CA, and L-OR) against H-OR bees, with terms reduced as in (A). (C) Regional CT_MIN comparisons (L-CA_MIN, H-CA_MIN, and L-OR_MIN) against H-OR_MIN bees. GO terms were summarized with ReViGO but did not require further filtering for presentation clarity. Complete sets of GO terms for all edgeR/Limma and WCGNA results are provided in Datasets S1 and S2, respectively.